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rabbit anti-cd34 pabs  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rabbit anti-cd34 pabs
    Rabbit Anti Cd34 Pabs, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-cd34+pabs/cd34+antibody/pm28828642-27-11-18
    Average 90 stars, based on 1 article reviews
    rabbit anti-cd34 pabs - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Detection of intrathrombotic endothelial progenitor cells and its application to thrombus age estimation in a murine deep vein thrombosis model.
    Article Snippet: Endothelial progenitor cells (EPCs), a newly identified cell type, are bone marrow-derived progenitor cells that co-express stem cell markers and Flk-1 (one of the receptors for vascular endothelial growth factor).. In this study, doublecolor immunofluorescence analysis was performed using antiCD34 and anti-Flk-1 antibodies in order to examine the timedependent intrathrombotic appearance of EPCs, using the thrombi of DVT model mice with different thrombus ages (1–21 days).. In thrombus cross-section specimens with an age of less than 3 days, CD34+/Flk-1+ EPCs were not detected.



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    A: Western blotting was used to detect the expression of CD31, <t>CD34</t> and VEGF proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of CD31, CD34 and VEGF proteins expression. C: immunohistochemical assay was used to detect the expression of CD31 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of CD34 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: immunohistochemical assay was used to detect the expression of VEGF protein in model group, cisplatin group and SSPH Ⅰ groups. E1: model group; E2: cisplatin group; E3: SSPH Ⅰ (25 mg/kg) group; E4: SSPH Ⅰ (50 mg/kg) group; E5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. F: quantitative analysis of CD31, CD34 and VEGF proteins expression. G: enzyme linked immunosorbent assay was used to detect the expression of VEGF protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); VEGF: vascular endothelial growth factor; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.
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    A: Western blotting was used to detect the expression of CD31, <t>CD34</t> and VEGF proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of CD31, CD34 and VEGF proteins expression. C: immunohistochemical assay was used to detect the expression of CD31 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of CD34 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: immunohistochemical assay was used to detect the expression of VEGF protein in model group, cisplatin group and SSPH Ⅰ groups. E1: model group; E2: cisplatin group; E3: SSPH Ⅰ (25 mg/kg) group; E4: SSPH Ⅰ (50 mg/kg) group; E5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. F: quantitative analysis of CD31, CD34 and VEGF proteins expression. G: enzyme linked immunosorbent assay was used to detect the expression of VEGF protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); VEGF: vascular endothelial growth factor; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.
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    A: Western blotting was used to detect the expression of CD31, CD34 and VEGF proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of CD31, CD34 and VEGF proteins expression. C: immunohistochemical assay was used to detect the expression of CD31 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of CD34 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: immunohistochemical assay was used to detect the expression of VEGF protein in model group, cisplatin group and SSPH Ⅰ groups. E1: model group; E2: cisplatin group; E3: SSPH Ⅰ (25 mg/kg) group; E4: SSPH Ⅰ (50 mg/kg) group; E5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. F: quantitative analysis of CD31, CD34 and VEGF proteins expression. G: enzyme linked immunosorbent assay was used to detect the expression of VEGF protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); VEGF: vascular endothelial growth factor; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.

    Journal: Journal of Traditional Chinese Medicine

    Article Title: Saponin Ⅰ from Shuitianqi ( Rhizoma Schizocapasae Plantagineae ) inhibits metastasis by negatively regulating the transforming growth factor-β1/Smad7 network and epithelial-mesenchymal transition in the intrahepatic metastasis Bagg’s Albino/c mouse model

    doi: 10.19852/j.cnki.jtcm.2024.04.006

    Figure Lengend Snippet: A: Western blotting was used to detect the expression of CD31, CD34 and VEGF proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of CD31, CD34 and VEGF proteins expression. C: immunohistochemical assay was used to detect the expression of CD31 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of CD34 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: immunohistochemical assay was used to detect the expression of VEGF protein in model group, cisplatin group and SSPH Ⅰ groups. E1: model group; E2: cisplatin group; E3: SSPH Ⅰ (25 mg/kg) group; E4: SSPH Ⅰ (50 mg/kg) group; E5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. F: quantitative analysis of CD31, CD34 and VEGF proteins expression. G: enzyme linked immunosorbent assay was used to detect the expression of VEGF protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); VEGF: vascular endothelial growth factor; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.

    Article Snippet: Subsequently, sections were incubated in 5% BSA blocking fluid for 1 h, with subsequent ON incubation in primary antibodies at 4 ℃: E-cadherin, N-cadherin, Vimentin, TGF-β1(PTG, 21898-1-AP), Smad7, MMP-2, MMP-9, CD31, CD34 (Sino Biological Inc., 50589-T24), and VEGF (all dilution 1:200).

    Techniques: Western Blot, Expressing, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay, Saline, Injection, Standard Deviation